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Updated: Sep 28, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Molecular characterization of a novel victorivirus infecting Corynespora cassiicola
Mingming Liu1,2, Yunxia Ni1, Hui Zhao1
1Institute of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, 450002, Zhengzhou, Henan, China.
Abstract:
A novel victorivirus was detected in an isolate of Corynespora cassiicola strain 20180909-03 and was named "Corynespora cassiicola victorivirus 1" (CcVV1). The complete genome sequence of this virus is 5140 bp in length and contains 57% GC with two large open reading frames (ORFs) overlapping at the tetranucleotide AUGA. The ORFs were predicted to encode a coat protein (CP) and an RNA-dependent RNA polymerase (RdRp), respectively, which are conserved in dsRNA fungal viruses of the family Totiviridae. Comparison and phylogenetic analysis of the deduced amino acid sequences of RdRp and CP showed that CcVV1 is a new member of the genus Victorivirus. This is the first report of a genomic sequence of a victorivirus infecting Corynespora cassiicola.
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