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Updated: Sep 28, 2025

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

Methods to evaluate virus-mediated acute lung inflammation.

Hannah M Ivester¹, Juselyn D Tupik², Margaret A Nagai-Singer²

¹Graduate Program in Translational Biology, Medicine, and Health, Virginia Polytechnic Institute and State University, Roanoke, VA, United States.

Methods in Cell Biology

|April 3, 2022

Summary

This summary is machine-generated.

Standardized airway tissue harvest protocols for mouse models of respiratory viral infections are presented. These methods maximize data acquisition for studying host immune responses and viral pathogenesis.

Keywords:

Lung inflammation Macrophage Neutrophil Pulmonary infection Respiratory virus

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Area of Science:

Immunology
Virology
Animal Models

Background:

Emerging infectious viruses necessitate standardized methods for studying respiratory illnesses.
Animal models are crucial for understanding host immune responses and viral pathogenesis kinetics.
Current methods for airway tissue harvest in animal models require optimization for data acquisition.

Purpose of the Study:

To present two distinct, standardized airway tissue harvest protocols for intranasal infection mouse models.
To enable comprehensive analysis of host immune responses and viral pathogenesis.
To maximize data acquisition and facilitate integration into standard laboratory workflows.

Main Methods:

Intranasal inoculation of mice with respiratory viruses.
Detailed description of two parallel tissue harvest protocols.
Integration of standard laboratory techniques for sample analysis.

Main Results:

The protocols allow for detailed investigation of host immune response and viral pathogenesis.
Standardized techniques ensure data consistency and comparability across studies.
The methods are adaptable for researchers without specialized training.

Conclusions:

The presented protocols offer a standardized approach to airway tissue harvest in mouse models.
These methods enhance data acquisition for studying respiratory viral infections.
The protocols provide flexibility for researchers to choose the best approach for their specific research questions.