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Updated: Sep 28, 2025
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Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
Published on: August 29, 2012
15.9K
Mouse models of graft-versus-host disease
Dilan A Patel1, Mark A Schroeder1, Jaebok Choi1
1Bone Marrow Transplantation & Leukemia, Oncology Division, Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, United States.
Methods in Cell Biology
|April 3, 2022
Summary
Allogeneic hematopoietic stem cell transplantation (HSCT) can cure blood diseases but may cause graft-versus-host disease (GVHD). This chapter details mouse models for studying acute and chronic GVHD to improve patient outcomes.
Area of Science:
- Hematology
- Immunology
- Transplantation Medicine
Background:
- Allogeneic hematopoietic stem and progenitor cell transplantation (allo-HCT) offers a cure for hematologic diseases.
- Graft-versus-host disease (GVHD) is a major complication of allo-HCT, mediated by donor T-lymphocytes.
- Both acute and chronic GVHD have significant morbidity and mortality, with current treatments like corticosteroids having limitations.
Purpose of the Study:
- To describe established mouse models for studying allo-HCT.
- To detail protocols for inducing and analyzing acute and chronic GVHD in these models.
- To facilitate the study of GVHD pathogenesis and the development of novel therapies.
Main Methods:
- Utilizing murine models to recapitulate key features of human allo-HCT.
- Implementing specific protocols to induce and monitor acute and chronic GVHD.
- Employing these models for preclinical drug development and mechanistic studies.
Main Results:
- Established mouse models accurately reflect human GVHD pathophysiology.
- These models allow for the investigation of distinct biological mechanisms underlying acute and chronic GVHD.
- The described protocols enable consistent induction and assessment of GVHD.
Conclusions:
- Mouse models are crucial for understanding GVHD and developing new treatments.
- Standardized protocols are essential for reproducible research in GVHD.
- Advancing GVHD prevention and therapy relies on continued research using these preclinical models.
