Toxic effect of triphenyl phosphate (TPHP) on Cyprinus carpio and the intestinal microbial community response

Triphenyl phosphate (TPHP) is a kind of organophosphorus flame retardants, and its use is increasing annually. However, the toxic effect associated with exposure to it has not been adequately investigated. Therefore, in this study, we determined the toxic dose of TPHP in the economic fish species, Cyprinus carpio. Acute and subacute toxicity tests were conducted, and the enrichment of TPHP in the gills, brain, intestines, and liver were determined by Liquid Chromatography-Mass Spectrometry, and the response of carp gut microbial community to TPHP stress was determined using 16 S rRNA gene high-throughput sequencing. Results showed that the 96-h LC₅₀ of TPHP in carp was 7 mg/L. At the 7 d, the order of TPHP absorption was as follows (from highest to lowest): gills > intestine > liver > brain, but at the 28 d and the purification period, the order of TPHP absorption was brain > gills > intestine > liver. TPHP exposure at 3.5 mg/L decreased α-diversity of the intestinal microbial community (p < 0.05), and altered community composition, in particular the relative abundance of dominant microbial populations. Functional profiles of the microbial communities predicted based on 16 S rRNA gene data showed upregulation in the degradation of exogenous substances and energy metabolism of the TPHP-treated groups (p < 0.05), suggesting that intestinal microbial taxa play a role in reducing TPHP toxicity. The results provide insights that could facilitate risk assessments of TPHP pollutants in aquatic environments and the management of associated water pollution.