Electron transfer capacity of humic acid in soil micro and macro aggregates in response to mulching years

Plastic film mulching can help farmers meet food production requirements and even increase output. Although the environmental impact of this mulch has received attention, uncertainty remains about certain soil components and the course of its long-term effects. In particular, it is not clear whether the long-term use of mulching film will affect the electron transfer capacity (ETC) of natural organic matter in the soil. This study evaluated the electron-accepting capacity (EAC) and electron-donating capacity (EDC) of soil humic acid (HA) in different-size aggregates in response to different film mulching years (0-6 years). The EAC of HA in the soil showed a downward trend as mulching years increased, while the EDC fluctuated. EAC decline in microaggregates (MIA) was more significant than that of macroaggregates (MAA). Film mulching changes the physical and chemical properties of soil and the activity of enzymes, changes the chemical structure of HA, and ultimately affects HA electron transfer. In addition, compared with that in MAA, the chemical structure of soil HA in MIA has a stronger correlation with enzyme activity and ETC and thus is more significantly affected by mulching. These results provide an in-depth understanding of the role of HA in soil aggregates of different sizes in processes related to the agricultural soil environment under mulching conditions.