An odorant receptor of the green mirid bug, Apolygus lucorum, tuned to linalool

A highly sensitive olfactory system is required for various insect behaviors, including oviposition site selection, host location, and mate recognition. Odorant receptors (ORs) play a critical role in odorant detection. In this study, we cloned four OR genes referred to as AlucORs (AlucOR4, AlucOR39, AlucOR43, and AlucOR47) from the green mirid bug, Apolygus lucorum, and used Real-time quantitative PCR to show that expression of all four ORs was considerably biased to antennae. Functional analysis, performed using a Xenopus oocyte expression system, revealed that AlucOR47 was robustly and sensitively tuned to the important plant volatile, linalool, and its analogs, linalyl acetate and linalool tetrahydride. Electroantennogram recordings showed that all three ligands elicited obvious responses in male and female mirid bug antennae, with the response to linalool being the strongest. In behavioral assays, male and female mirid bugs displayed significant aversions to linalool. Additionally, the repellent behavior effect of A. lucorum in response to linalool disappeared after knocking down AlucOR47 by RNA interference (RNAi). Taken together, these results indicate that AlucOR47 is necessary for linalool perception in A. lucorum. Our results suggest that AlucOR47 may play a role in plant-insect interactions and provide insight into potential means of biological control against mirid bugs.