Suppression of PBAN receptor expression reduces fecundity in the fall armyworm, Spodoptera frugiperda

The fall armyworm, Spodoptera frugiperda, native to the tropical and subtropical areas of the American continent is one of the world's most destructive insect pests. In most insects, sex pheromone production is initiated following the activation of a pheromone-biosynthesis-activating neuropeptide (PBAN) receptor, which belongs to G protein-coupled receptor. We explored expression level of S. frugiperda PBAN receptor (Sf-PBANr) gene and validated the physiological function by assessing the fecundity of adult females subjected to its specific RNA interference (RNAi). Sf-PBANr was predicted from a transcriptome of S. frugiperda. Reverse-transcription polymerase chain reaction assay showed its expression in all developmental stages of S. frugiperda. Specific suppression of Sf-PBANr by RNAi in either sex significantly reduced the total number of laid eggs per adult female. Matings between both RNAi-treated males and female resulted in 63.3% reduction in fecundity. In contrast, the RNAi effect was less 47.5%-49.5% at the matings from single-parent RNAi treatment. These results suggest that the Sf-PBANr is associated with female of S. frugiperda.