Potential Lignocellulolytic Microfungi from Pineapple Plantation for Composting Inoculum Additive

Area of Science:

• Microbiology
• Biotechnology
• Environmental Science

Background:

• Pineapple plantations generate substantial lignocellulose-rich biomass, posing decomposition challenges.
• Natural decomposition of pineapple residue is hindered by its recalcitrant lignocellulosic structure.

Purpose of the Study:

• To isolate and characterize lignocellulolytic microfungi from pineapple plantations.
• To identify potential candidates for developing stress-resistant inoculums for pineapple litter composting.

Main Methods:

• Isolation of microfungi from pineapple litter and soil using selective media.
• Characterization of cellulolytic, xylanolytic, and ligninolytic activities of fungal isolates.
• Assessment of spore productivity and viability for selected isolates.

Main Results:

• Eleven dominant lignocellulolytic microfungi were identified.
• Five cellulolytic (Bioggp 3, 6, 9, 11, 12) and five xylanolytic (Bioggp 3, 6, 8, 9, 12) isolates were selected.
• Bioggp 3 exhibited the highest cellulolytic (4.0) and xylanolytic (4.20) indices. Bioggp 5 showed stronger ligninolytic activity (1.47 zone ratio).
• High spore productivity and viability were observed for isolates Bioggp 9 (cellulolytic), Bioggp 3 (xylanolytic), and Bioggp 5 (ligninolytic).

Conclusions:

• The isolated microfungi possess significant lignocellulolytic capabilities.
• These findings support the development of fungal inoculums for efficient pineapple litter composting.
• The identified microfungi can contribute to sustainable waste management in pineapple agroecosystems.