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Bachu Mushroom Polysaccharide Alleviates Colonic Injury by Modulating the Gut Microbiota
Zumrat Abdureyim1, Lei Wang2,3, Qing Tao2
1Department of Labor Hygiene and Environmental Hygiene, College of Public Health, Xinjiang Medical University, Urumqi, 830017 Xinjiang, China.
Objective:
This study was to define the protective effect of purified Helvella leucopus polysaccharide (p-HLP) against dextran sulfate sodium- (DSS-) induced colitis.
Methods:
The novel p-HLP was isolated from Bachu mushroom through hot water extraction, ethanol precipitation, and column chromatography. Then, we evaluated the potential effects of p-HLP on colonic histopathology, inflammation, and microbiota composition in DSS-induced colitis mice.
Results:
p-HLP was a homopolysaccharide with an average molecular weight of 39.14 × 108 Da. Functionally, p-HLP significantly attenuated DSS-induced body weight loss and colon shortening. The histological score of the colon lesion was significantly decreased upon p-HLP treatment. Also, p-HLP treatment led to decreased expression of proinflammatory cytokines and mediators (IL-6, IL-1β and TNF-α, and COX-2 and iNOS) and increased expression of anti-inflammatory cytokine (IL-10) in the colon tissues. Illumina MiSeq sequencing revealed that p-HLP modulated the composition of the gut microbiota.
Conclusion:
p-HLP is a potent regulator that protects the lesions from DSS-induced colitis.
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