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Updated: Sep 28, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
Published on: July 6, 2022
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
Yang Liu1,2, Marcello DiStasio3, Graham Su1,2
1Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA.
None:
We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

