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Updated: Aug 26, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
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Published on: June 14, 2014

Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids

B P Dorman, N Shimizu, F H Ruddle

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1978
    PubMed

    Somatic cell hybrids of human fibroblasts and mouse A9 cells, carrying only a portion of the human X chromosome in a mouse chromosome background, were injected into C3H mice. The resulting mouse anti-hybrid cell antisera contain antibodies found to be human specific and to react with only those hybrid cells carrying the human X chromosome, as confirmed by essentially perfect concordance between antibody binding assayed by indirect immunofluorescence and presence of the human X-linked enzyme hypoxanthine phosphoribosyl-transferase determined by autoradiographic assay of [3H] hypoxanthine utilization. Heterogeneous mixtures of hybrid cells may be analyzed into fluorescent (X plus) and nonfluorescent (X minus) subpopulations and fractionated viably by using a fluorescence-activated cell sorter.

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