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Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds
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Wound fluid sampling methods for proteomic studies: A scoping review.

Joe Harvey1,2, Kieran T Mellody1, Nicky Cullum2,3

  • 1Centre for Dermatology Research, School of Biological Sciences, The University of Manchester & Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, UK.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society
|April 5, 2022
PubMed
Summary
This summary is machine-generated.

This review maps wound fluid sampling methods, finding vacuum and drainage techniques most common. Methodological limitations like small sample sizes hinder biomarker discovery for chronic wound healing.

Keywords:
complex woundsurgical woundwound biomarkerwound fluid collectionwound proteomics

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Area of Science:

  • Wound healing research
  • Biomarker discovery
  • Proteomics

Background:

  • Effective wound care and treatment development rely on understanding non-healing wounds.
  • Accurate wound fluid analysis is crucial, but sampling methods can impact results.
  • Standardization of wound fluid collection is needed for reliable protein profiling.

Purpose of the Study:

  • To systematically review and map current methods for collecting wound fluid for protein analysis.
  • To identify common techniques and sample sources used in wound fluid research.
  • To highlight limitations in current sampling methodologies.

Main Methods:

  • A comprehensive literature search was conducted in MEDLINE, Embase, and CINAHL Plus (May 2020).
  • Eligibility screening involved one reviewer with consensus from a second reviewer.
  • Included studies were quantitatively mapped and summarized narratively.

Main Results:

  • 280 studies were included in this scoping review.
  • Vacuum, drainage, or external devices were the most frequent collection methods.
  • Surgical wounds were the most common sample source; absorbent materials and occlusive dressings were also utilized.

Conclusions:

  • Significant variability exists in wound fluid collection methods.
  • Small sample sizes and short collection periods limit biomarker discovery and validation.
  • Future research should focus on reproducible methods for larger longitudinal studies.