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Updated: Sep 28, 2025
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells
Published on: September 20, 2019
Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells
Yibin Zhang1, Dilka Liyana Arachchige1, Adenike Olowolagba1
1Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA.
Abstract:
A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.
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