Related Experiment Video

Updated: Sep 28, 2025

Artificial Antigen Presenting Cell aAPC Mediated Activation and Expansion of Natural Killer T Cells
13:18

Artificial Antigen Presenting Cell aAPC Mediated Activation and Expansion of Natural Killer T Cells

Published on: December 29, 2012

18.3K

Changes in the natural killer cell repertoire and function induced by the cancer immune adjuvant candidate

Helen Y Lorenzo-Anota1, Alan B Martínez-Loria1, Reyes S Tamez-Guerra2

  • 1Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Mexico; Unité de Biologie Cellulaire des Lymphocytes, Institut Pasteur, Paris, France.

Cellular Immunology
|April 5, 2022
PubMed

Insights

IMMUNEPOTENT-CRP (I-CRP), a bovine extract, enhances natural killer (NK) cell activity. This extract modifies NK cell receptors, boosting their ability to eliminate target cells without causing proliferation.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Natural killer (NK) cells are crucial immune cells that target virus-infected, malignant, and antibody-coated cells.
  • NK cells are characterized as CD3-, CD16+, CD56+.

Purpose of the Study:

  • To investigate the effects of IMMUNEPOTENT-CRP (I-CRP), a bovine dialyzable leukocyte extract (DLE), on human NK cell activation, receptor repertoire, and effector functions.
  • To determine if I-CRP influences NK cell proliferation.

Main Methods:

  • Human NK cells from normal donors were treated with I-CRP.
  • Flow cytometry was used to analyze changes in NK cell surface receptors (NKp30, NKp44, NKp46, NKG2D, NKG2C, KIR, CD160, CD85j, CD226) and subsets (CD56Dim CD16-).
  • Degranulation activity was assessed using the CD107a assay, and cytotoxicity was measured via calcein release assay against K562 target cells.

Main Results:

  • I-CRP treatment activated human NK cells and increased the CD56Dim CD16- subset, without inducing proliferation.
  • Significant increases were observed in NK cell receptors NKp30, NKp44, NKp46, NKG2D, NKG2C, and KIR.
  • I-CRP-treated NK cells showed enhanced degranulation and increased cytotoxicity against K562 cells.

Conclusions:

  • I-CRP modifies the human NK cell receptor repertoire.
  • This modification leads to enhanced NK cell cytotoxic activity.
  • I-CRP demonstrates potential as an agent to stimulate NK cells.
Keywords:
Cell activationDialyzable leukocyte extractEffector activityImmunotherapyNK cellNatural killer cellsReceptors repertoire

More Related Videos

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

18.9K
Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells11:08

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Published on: February 14, 2025

1.1K

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
3.0K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
4.4K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
3.9K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
691