Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations between Meal Timing and Frequency and Obesity among Adults in Jeju, Republic of Korea.

Preventive nutrition and food science·2026
Same author

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
Same author

AI-driven tripartite classification for optimizing wearable bioelectronics in depression management.

Science advances·2026
Same author

Targeting the senescence‒autophagy axis via p16<sup>INK4a</sup> inhibition alleviates pulmonary fibrosis.

Signal transduction and targeted therapy·2026
Same author

Identification of Anti-Viral Compounds from <i>Toxicodendron vernicifluum</i> Extract That Inhibit the Coronavirus Replication.

Journal of microbiology and biotechnology·2026
Same author

Chemical insights on covalent organic frameworks for ferroptosis and pyroptosis in cancer.

Chemical Society reviews·2026
Same journal

Cortical Brain Entropy Architecture Reveals Multidimensional Structure of Schizophrenia.

Biophysical reports·2026
Same journal

The oligomeric state of chitooligosaccharide deacetylase from the marine bacterium Vibrio campbellii.

Biophysical reports·2026
Same journal

Quantifying Species-Specific Binding Affinities of Transthyretin Aggregation Inhibitors.

Biophysical reports·2026
Same journal

Drosophila jump muscle myofibrils: A new tool for investigating activation and relaxation.

Biophysical reports·2026
Same journal

Optical tweezers combined with FRET tension sensor reveal force-dependent vinculin dynamics.

Biophysical reports·2026
Same journal

Role of E. coli acid resistance systems in proton motive force formation during fermentation.

Biophysical reports·2026
See all related articles

Related Experiment Video

Updated: Sep 28, 2025

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.6K

A mean shift algorithm for drift correction in localization microscopy.

Frank J Fazekas1, Thomas R Shaw1,2, Sumin Kim3

  • 1Program in Biophysics, University of Michigan, Ann Arbor, Michigan.

Biophysical Reports
|April 6, 2022
PubMed
Summary
This summary is machine-generated.

Sample drift in single-molecule localization microscopy (SMLM) can compromise image quality. This study introduces an efficient peak-finding algorithm to accurately estimate and correct drift in 2D and 3D SMLM data.

More Related Videos

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.8K
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.5K

Related Experiment Videos

Last Updated: Sep 28, 2025

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.6K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.8K
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.5K

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Single-molecule localization microscopy (SMLM) achieves super-resolution by localizing individual fluorescent molecules.
  • Long acquisition times required for SMLM can lead to significant sample drift.
  • Sample drift can degrade image quality and reduce localization precision.

Purpose of the Study:

  • To develop an efficient and robust method for estimating sample drift in SMLM.
  • To provide a solution for improving the accuracy of SMLM data affected by drift.

Main Methods:

  • Implementation of a peak-finding algorithm based on mean-shifts.
  • Application of the algorithm for drift estimation in 2D and 3D SMLM datasets.
  • Validation of the method's effectiveness for SMLM.

Main Results:

  • The proposed method efficiently estimates drift in SMLM.
  • The algorithm is robust and effective in both two and three dimensions.
  • Accurate drift estimation improves the reliability of SMLM data.

Conclusions:

  • The developed mean-shift peak-finding algorithm offers an effective solution for drift correction in SMLM.
  • This method enhances the precision and quality of super-resolution images obtained via SMLM.
  • The technique is broadly applicable to various SMLM modalities.