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Updated: Sep 28, 2025

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
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Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

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Hsa_circ_0030042 Facilitates the Proliferation and Migration of Vascular Smooth Muscle Cells via the

Ji Ma1, Jia Liu2, Tengfei Li1

  • 1Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists
|April 6, 2022
PubMed

Insights

Circular RNA hsa_circ_0030042 promotes atherosclerosis by increasing vascular smooth muscle cell proliferation and migration. Targeting this circRNA may offer a new therapeutic strategy for atherosclerosis treatment.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Circular RNAs (circRNAs) are implicated in atherosclerosis (AS) pathogenesis.
  • Vascular smooth muscle cells (VSMCs) play a key role in AS progression.
  • The precise mechanisms of circRNA regulation in VSMC behavior are not fully understood.

Purpose of the Study:

  • To investigate the role of circRNAs in regulating VSMC proliferation and migration in AS.
  • To elucidate the molecular mechanism underlying circRNA function in AS.

Main Methods:

  • Established an AS cell model using tumor necrosis factor-alpha (TNF-α) treated VSMCs.
  • Assessed cell proliferation, apoptosis, and migration using CCK-8, TUNEL, and Transwell assays.
  • Investigated molecular interactions via RNA immunoprecipitation, RNA pull-down, and luciferase reporter assays.

Main Results:

  • Identified a novel circRNA, hsa_circ_0030042, upregulated in TNF-α-induced VSMCs.
  • Silencing hsa_circ_0030042 reduced VSMC proliferation and migration while enhancing apoptosis.
  • Demonstrated that hsa_circ_0030042 positively regulates forkhead box O1 (FOXO1) expression by sponging miR-514a-3p.

Conclusions:

  • The hsa_circ_0030042/miR-514a-3p/FOXO1 axis is crucial in AS progression.
  • This study enhances the understanding of circRNA's role in atherosclerosis.
Abstract

Keywords:
FOXO1atherosclerosishsa_circ_0030042vascular smooth muscle cells

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