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Sensitive Electrochemiluminescence Biosensor Based on the Target Trigger Difference of the Electrostatic Interaction
Yilei Lu1, Xiujun Rong1, Peng Wu2
1Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
Analytical Chemistry
|April 6, 2022
Summary
This study introduces a novel electrochemiluminescence (ECL) biosensor for detecting human papillomavirus 16 (HPV 16) DNA. The sensor utilizes electrostatic interactions to achieve highly sensitive detection of HPV 16 DNA.
Area of Science:
- Electrochemistry
- Nanotechnology
- Biosensing
Background:
- Developing sensitive and specific biosensors is crucial for early disease diagnosis.
- Electrochemical and electrochemiluminescence (ECL) methods offer promising avenues for biosensing applications.
- Detecting human papillomavirus 16 (HPV 16) DNA is vital for cervical cancer screening.
Purpose of the Study:
- To develop a novel ECL biosensor for the detection of HPV 16 DNA.
- To leverage the discrepancy in electrostatic interactions for signal generation and amplification.
- To establish a sensitive and reliable method for HPV 16 DNA quantification.
Main Methods:
- Modification of a gold electrode surface with short single-stranded capture probes (CP).
- Utilizing tris(2,2'-bipyridyl) ruthenium(II) chloride hexahydrate-doped SiO2 nanoparticles (Ru@SiO2 NPs) as signal generators.
- Employing hybrid chain reaction (HCR) amplification for target DNA.
- Exploiting electrostatic interactions and steric hindrance for signal modulation.
Main Results:
- A strong ECL signal was observed in the absence of target DNA due to weak electrostatic interaction.
- The presence of target HPV 16 DNA led to enhanced negative charge on the electrode, reducing ECL signal.
- The ECL signal decrease showed a linear correlation with the logarithm of HPV concentration.
- Achieved a wide detection range (0.1 fM - 5 pM) with an ultra-low limit of detection (1.41 aM).
Conclusions:
- The developed ECL biosensor effectively detects HPV 16 DNA by exploiting electrostatic interactions.
- This innovative methodology expands the application of electrostatic interactions in ECL sensing.
- The platform can be adapted for the detection of other DNA targets by modifying the probe sequence.
