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High-Force Application by a Nanoscale DNA Force Spectrometer.

Michael Darcy, Kyle Crocker, Yuchen Wang

  • 1Department of Chemistry, University of Sheffield, Sheffield S3 7HF, U.K.

ACS Nano
|April 6, 2022
PubMed
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DNA origami nanocalipers can now apply high forces (>20 pN) at the nanoscale. This breakthrough advances nanomedicine and molecular robotics by enabling precise force measurements in confined biological environments.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • High force application (>10 pN) at the nanoscale is crucial for nanomedicine, molecular robotics, and understanding biological processes.
  • Existing techniques like atomic force microscopy have limitations in constrained environments.
  • DNA-based molecular calipers offer a promising alternative but are limited to low forces.

Purpose of the Study:

  • To develop and characterize DNA origami nanocalipers with tunable mechanical properties for high force application.
  • To quantify the force output and dynamic range of these DNA devices.
  • To enable new applications in nanobiotechnology and molecular studies.

Main Methods:

  • Implementation of DNA origami nanocalipers designed to rupture DNA duplexes.
Keywords:
DNA origami nanotechnologyDNA shearingforce spectroscopypartition function modelingsingle-molecule fluorescence

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  • Integration of static and dynamic single-molecule characterization techniques.
  • Application of statistical mechanical modeling to quantify device properties.

    • Main Results:

    • The DNA origami nanocalipers demonstrated the ability to apply forces exceeding 20 piconewtons.
    • A nanometer-scale dynamic range was achieved.
    • Thermally driven dynamics were key to the device's force application capabilities.

    Conclusions:

    • DNA origami nanocalipers represent a significant advancement for nanoscale force spectroscopy.
    • These devices overcome limitations of existing methods in confined geometries.
    • Potential applications include studying protein unfolding and controlling molecular interactions in nanodevices.