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Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
Recent patents in allergy and immunology: A quantitative real-time polymerase chain reaction method for diagnosing
Pureun-Haneul Lee1, SeonMuk Choi1, MinHyeok An1
1Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
Background:
Asthma is diagnosed based on a history of the characteristic symptoms and evidence of expiratory airflow limitation. However, asthma diagnosis using the existing tests is associated with a risk of coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 spread. In this study, we developed a quantitative real-time polymerase chain reaction (qRT-PCR)-based asthma diagnosis tool.
Methods:
We detected nectin-4 in the plasma of patients with asthma using qRT-PCR, explored the relationship of clinical variables.
Results:
quantitative real-time polymerase chain reaction revealed that plasma nectin-4 mRNA levels were higher in asthmatics than controls. These results correlated with lung function.
Conclusions:
Those results suggest that qRT-PCR for nectin-4 may aid asthma diagnosis and monitoring.
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