Related Experiment Video
Updated: Jul 26, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Published on: April 2, 2012
Absence of Herpesvirus DNA in Aqueous Humor from Asymptomatic Subjects
Joanna von Hofsten1,2, Tomas Bergström3, Madeleine Zetterberg1,4
1Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SE-405 30, Sweden.
Purpose:
To assess herpesvirus DNA detection in aqueous humor from a cohort of asymptomatic Scandinavian patients undergoing elective cataract surgery.
Patients And Methods:
Prospective case series. Aqueous samples were obtained from 30 patients undergoing elective cataract surgery. Polymerase chain reaction (PCR) analysis for herpes simplex virus 1 (HSV1), herpes simplex virus 2 (HSV2), cytomegalovirus (CMV), Epstein Barr virus (EBV) was performed. Toxoplasma was added to the analysis due to its role as pathogen with ocular latency.
Results:
Mean age of participants was 75.3 years. Sixteen subjects (53%) had ocular comorbidities. Five subjects (17%) had endothelial dysfunction without known hereditary pattern. None of the samples were positive for herpesviruses or toxoplasma.
Conclusion:
None of the aqueous samples were positive, suggesting shedding does not frequently occur in the aqueous humor of asymptomatic patients.
More Related Videos
13:22Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
Published on: January 23, 2014
06:20Author Spotlight: Advancing VRL Diagnosis Using Cell-Free DNA Extraction from Vitreous Humor
Published on: January 12, 2024
Related Concept Videos
Herpes
Poliomyelitis
Cytomegalovirus Disease
Genital Herpes