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Published on: December 4, 2015
Evolutionary analysis of 3 isolates of Seneca Valley virus from a single pig farm
Jianguo Dong1, Dan Rao1, Xin Geng1
1School of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China (Dong, Rao, Chen, He, Jiao, Huang, Chen); Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510633, China (Cong); Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China (Zhao); College of Animal Science and Technology, Jilin Agriculture University, Changchun 130018, China (Geng).
Abstract:
Vesicular disease caused by Seneca Valley virus (SVV) has recently emerged throughout China and caused certain industry losses. We used immunofluorescence and western blotting to confirm that 3 new SVV strains (CH-GDSG-2018-1, CH-GDSG-2018-2, and CH-GDSG-2018-3) were from 1 pig farm. Phylogenetic analysis revealed the following: i) all 3 strains belong to USA-GBI29-2015-like clades, ii) CH-GDSG-2018-3 might have diverged from CH-GDSG-2018-1 and CH-GDSG-2018-2, and iii) CH-GDSG-2018-3 is a recombinant of the CHhb17 and HeNKF-1 strains. Virus growth curves showed that CH-GDSG-2018-3 had stronger proliferation ability in vitro. Seneca Valley virus has evolved extensively within China and this study has furthered our understanding of SVV epidemiology.
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