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ADAM19 and TUBB1 Correlate with Tumor Infiltrating Immune Cells and Predicts Prognosis in Osteosarcoma
Jun Wang1, Mingzhi Gong1, Zhenggang Xiong1
1Department of Orthopedics and Trauma, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, China.
Combinatorial Chemistry & High Throughput Screening
|April 7, 2022
Summary
This study identified ADAM19 and TUBB1 as key prognostic genes in osteosarcoma, with TUBB1 showing potential as a multi-drug target for therapy. Their expression correlates with immune cell infiltration in bone tumors.
Area of Science:
- Oncology
- Genomics
- Immunology
Background:
- Osteosarcoma is the most common primary malignant bone tumor.
- Identifying prognostic markers is crucial for improving patient outcomes.
Purpose of the Study:
- To identify key prognostic genes in osteosarcoma.
- To investigate the roles of these genes in tumor progression and immune cell infiltration.
Main Methods:
- Analysis of three GEO microarray datasets for osteosarcoma.
- Screening of differentially expressed genes (DEGs) using Limma.
- Functional enrichment analysis via DAVID, GeneMANIA, and Metascape.
- Survival analysis and CIBERSORT for immune cell infiltration assessment.
Main Results:
- Identified common up-regulated and down-regulated DEGs.
- ADAM19 and TUBB1 expression correlated with favorable prognosis.
- Discovered correlations between gene expression and infiltration of CD8 T cells, macrophages, and other immune cells.
- Identified 56 drugs targeting TUBB1.
Conclusions:
- ADAM19 and TUBB1 serve as potential prognostic biomarkers for osteosarcoma.
- Gene expression is linked to tumor-infiltrating immune cells.
- TUBB1 represents a potential therapeutic target due to its multi-drug targeting potential.