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Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
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Interfacing microfluidics with information-rich detection systems for cells, bioparticles, and molecules.
Jared P Smithers1, Mark A Hayes2
1School of Molecular Sciences, Arizona State University, Mail Stop 1604, Tempe, AZ, 85287, USA.
Analytical and Bioanalytical Chemistry
|April 7, 2022
Summary
This review details microfluidic "off-chipping" techniques for transferring tiny biological samples to advanced detection systems. It covers spraying, droplet, and phase-change methods for precise sample delivery.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Microfluidics
Background:
- Microfluidic systems enable precise manipulation of small sample volumes and biomaterials.
- Advanced detection methods like mass spectrometry and sequencing require efficient sample transfer from microfluidic devices.
Purpose of the Study:
- To review and organize advancements in microfluidic sample "off-chipping" techniques.
- To highlight methods for translating microfluidically processed samples to information-rich detection systems.
Main Methods:
- The review categorizes and discusses various off-chipping delivery techniques.
- Key publications illustrating advancements in each technique are analyzed.
Main Results:
- Techniques include spraying (electrospray, pneumatic), meniscus-defined volumes (droplets), constrained volumes, and phase changes (deposition, freezing).
- Each method effectively delivers highly defined samples from microfluidic systems for analysis.
Conclusions:
- Significant progress has been made in developing effective off-chip delivery methods for microfluidic samples.
- These advancements facilitate the use of sensitive, information-rich detection schemes for biomaterials.
