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RAMP2-AS1 inhibits CXCL11 expression to suppress malignant phenotype of breast cancer by recruiting DNMT1 and DNMT3B

Li Li1, Ya-Ping Gan2, Hui Peng3

  • 1Department of Breast Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, PR China.

Experimental Cell Research
|April 7, 2022
PubMed
Abstract

Insights

RAMP2-AS1, a long non-coding RNA, suppresses breast cancer progression by inhibiting CXCL11 expression. Decreased RAMP2-AS1 levels correlate with poor prognosis, highlighting its therapeutic potential in breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a prevalent malignancy affecting women globally.
  • Understanding the molecular mechanisms underlying breast cancer development is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the role of RAMP2-AS1 in breast cancer.
  • To elucidate the mechanism by which RAMP2-AS1 affects breast cancer progression, focusing on its interaction with CXCL11, DNMT1, and DNMT3B.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blot were used to assess RAMP2-AS1 and CXCL11 expression.
  • RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays confirmed interactions between RAMP2-AS1, DNMT1, and DNMT3B.
  • Functional assays (cell viability, proliferation, migration, apoptosis) and immunohistochemistry (IHC) evaluated the impact of RAMP2-AS1 on breast cancer phenotypes and tumor growth.

Main Results:

  • RAMP2-AS1 expression was significantly decreased in breast cancer tissues and cells, correlating with poor patient prognosis.
  • RAMP2-AS1 inhibited breast cancer cell proliferation, migration, and promoted apoptosis.
  • RAMP2-AS1 recruited DNMT1 and DNMT3B to the CXCL11 promoter, leading to its methylation and subsequent downregulation.

Conclusions:

  • RAMP2-AS1 acts as a tumor suppressor in breast cancer by inhibiting the expression of CXCL11 through epigenetic mechanisms involving DNMT1 and DNMT3B.
  • Restoring RAMP2-AS1 expression may represent a potential therapeutic strategy for breast cancer.
Keywords:
Breast cancerCXCL11DNMT1DNMT3BRAMP2-AS1

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