Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rationally designed copper-deferiprone-phenanthroline complex as a dual anticancer and SARS-CoV-2 inhibitor.

RSC advances·2026

Same author

Development of a terbium-based coordination polymer nanoprobe for determination of clonazepam in exhaled breath condensate.

BioImpacts : BI·2026

Same author

Missing-data-aware machine learning prediction of in-hospital major adverse cardiovascular events after primary percutaneous coronary intervention for ST-segment elevation myocardial infarction.

Scientific reports·2026

Same author

Correction: Synthesis of terbium and silica modified graphene quantum dots for targeted bioimaging of breast cancer cells using folate receptors.

Scientific reports·2026

Same author

Assessing valproate-induced changes in NADH levels in patients with type 2 diabetes using a silver nanoparticle-based method: An in-vitro study.

Journal of pharmaceutical and biomedical analysis·2026

Same author

Determination of fenofibric acid in saliva and exhaled breath condensate samples using metal- organic frameworks based dispersive micro solid phase extraction coupled to HPLC-PDA.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026

Search research articles

Related Experiment Video

Updated: Sep 27, 2025

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Published on: September 10, 2018

Quantification of methotrexate in plasma samples using highly fluorescent nanoparticles.

Zahra Golsanamlou¹, Houman Kholafazad-Kordasht², Jafar Soleymani³

¹Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Pharmaceutical and Biomedical Analysis

|April 7, 2022

Summary

A new amine-functionalized silica carbon polymer dots sensor detects methotrexate (MTX) in plasma. This sensitive and selective nanoprobe is suitable for therapeutic drug monitoring.

Keywords:

Fluorescence, plasma, real samples Methotrexate Silica carbon polymer dots

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Related Experiment Videos

Last Updated: Sep 27, 2025

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Published on: September 10, 2018

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Area of Science:

Materials Science
Analytical Chemistry
Nanotechnology

Background:

Methotrexate (MTX) is a crucial drug in cancer therapy, requiring precise monitoring.
Accurate quantification of MTX in biological samples is essential for effective therapeutic drug monitoring (TDM).
Existing methods for MTX detection may lack the sensitivity, selectivity, or simplicity required for routine clinical use.

Purpose of the Study:

To develop a novel optical sensor for sensitive and selective detection of methotrexate (MTX).
To utilize amine-functionalized silica carbon polymer dots (APTES-CPDs) as a core-shell nanostructure for fluorescence-based MTX sensing.
To evaluate the performance of the developed sensor for MTX determination in plasma samples for therapeutic drug monitoring.

Main Methods:

Synthesis of APTES-CPDs core-shell nanostructures via a one-step hydrothermal process.
Utilizing the fluorescence quenching effect of MTX on APTES-CPDs for optical sensing.
Optimizing reaction conditions and validating the sensor's performance using plasma samples.

Main Results:

The APTES-CPDs sensor demonstrated dual linear detection ranges for MTX: 10 ng/mL to 2.0 µg/mL and 2.0 µg/mL to 50 µg/mL.
Achieved low limits of detection (LLOD) of 10 ng/mL and 2.0 µg/mL in the respective linear ranges.
The sensor exhibited high accuracy, selectivity towards MTX over co-administered drugs, and applicability in real plasma samples.

Conclusions:

The developed APTES-CPDs-based nanoprobe offers a simple, highly selective, and sensitive method for MTX detection.
This sensor is suitable for routine therapeutic drug monitoring of methotrexate in plasma.
The findings highlight the potential of functionalized carbon polymer dots as advanced nanomaterials for biosensing applications.