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DNA -based hydrogels for high-performance optical biosensing application.

Paola Zezza1, María Isabel Lucío2, Ángel Maquieira2

  • 1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valéncia, Universitat de Valéncia, Camino de Vera s/n, 46022, Valencia, Spain.

Talanta
|April 7, 2022
PubMed
Summary

DNA-based hydrogels offer adaptable biosensor platforms. This study details their design and application for specific target sequence detection using novel immobilization techniques and advanced sensing formats.

Keywords:
Acrydite-modified DNADNA-Based hydrogelHolographic biograting hydrogelMicroarrayOptical biosensingUV photopolymerization

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Analyte-sensitive DNA-based hydrogels are versatile for biosensor applications.
  • Their adaptable nature allows for tailored recognition capabilities.

Purpose of the Study:

  • To present the design of a DNA-based hydrogel.
  • To demonstrate its application as a sensing platform for specific target sequence detection.

Main Methods:

  • DNA-functionalized hydrogel structures synthesized via free radical co-polymerization.
  • One-step probe immobilization by UV-initiated covalent attachment during hydrogel formation.
  • Utilizing specific nucleotide sequences for recognition ability.

Main Results:

  • Successful application of DNA-based hydrogels for high-performance target detection in fluorescence microarray format.
  • Fabrication of holographic surface relief gratings for label-free sensing assays.

Conclusions:

  • DNA-based hydrogels provide a robust and adaptable platform for biosensing.
  • The developed method enables efficient probe immobilization and versatile sensing applications.