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Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

216
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
216
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

477
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
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Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

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Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
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Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

343
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
343
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

481
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
481

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mtTB: A Web-Based R/Shiny App for Pulmonary Tuberculosis Screening.

Zhougui Ling1, Shuangping Huang1, Zhongwei Wen1

  • 1Department of Pulmonary and Critical Care Medicine, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China.

Frontiers in Cellular and Infection Microbiology
|April 8, 2022
PubMed
Summary

New mitochondria-derived small RNAs show promise as biomarkers for diagnosing pulmonary tuberculosis (TB). A novel prediction model accurately distinguishes TB patients from controls, aiding in blood-based screening and clinical diagnosis.

Keywords:
TBbiomarkersmitochondria-derived small RNAsperipheral bloodratio-based method

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Infectious Diseases

Background:

  • Pulmonary tuberculosis (TB) diagnosis remains a clinical challenge.
  • Small non-coding RNAs are potential TB biomarkers, but current detection methods lack stability.
  • Reliable biomarkers are needed for effective pulmonary tuberculosis screening.

Purpose of the Study:

  • To identify reliable biomarkers for pulmonary tuberculosis screening using mitochondria-derived small RNAs.
  • To develop a prediction model for discriminating TB patients from healthy controls.
  • To create a user-friendly tool for clinical application.

Main Methods:

  • Utilized a ratio-based method on mitochondria-derived small RNAs from peripheral blood mononuclear cells.
  • Developed a prediction model based on seven mtRNA biomarkers.
  • Validated the model in discovery and independent cohorts.
  • Created an R Graphical User Interface (GUI) tool named mtTB.

Main Results:

  • The seven mtRNA biomarker model achieved high discrimination in both discovery (AUC = 0.906) and validation (AUC = 0.968) cohorts.
  • The model successfully differentiated pulmonary tuberculosis patients from controls.
  • The mtTB R GUI offers a feasible approach for blood-based TB screening.

Conclusions:

  • Mitochondria-derived small RNAs are promising biomarkers for pulmonary tuberculosis diagnosis.
  • The developed prediction model and mtTB tool offer a more accurate and reliable method for clinical practice.
  • This approach facilitates blood-based screening for pulmonary tuberculosis.