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Updated: Sep 27, 2025
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke
Xuan Li1, Jingjun Lyu1, Ran Li1
1Multidisciplinary Brain Protection Program, Center for Perioperative Organ Protection, Department of Anesthesiology, Duke University Medical Center, P.O. Box 3094, Durham, NC, 27710, USA.
Background:
Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells.
Methods:
Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used.
Results:
We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles.
Conclusions:
This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.
