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Hsa_circ_0008344 Promotes Glioma Tumor Progression and Angiogenesis Presumably by Regulating miR-638/SZRD1 Pathway
Liyong Deng1, Kuiyu Gong1, Guihua Wang2
1Department of Oncology, Changsha Central Hospital, No. 161, Shaoshan South Road, Changsha, Hunan, China.
Neurotoxicity Research
|April 8, 2022
Summary
Hsa_circRNA_0008344 (circ_0008344) promotes glioma progression by sponging miR-638 and upregulating SZRD1. Silencing circ_0008344 inhibits glioma proliferation, migration, invasion, and angiogenesis, offering a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Glioma is a primary brain tumor with poor prognosis.
- Circular RNAs (circRNAs) are emerging as key regulators in various cancers, including glioma.
- The role of hsa_circRNA_0008344 (circ_0008344) in glioma progression remains largely unexplored.
Purpose of the Study:
- To investigate the functional role of circ_0008344 in glioma tumor progression.
- To elucidate the underlying molecular mechanism of circ_0008344 in glioma.
Main Methods:
- Quantitative PCR and Western blotting to assess RNA and protein levels.
- Cell proliferation, migration, invasion, and angiogenesis assays (CCK-8, EdU, Transwell, tube formation).
- Bioinformatics, dual-luciferase reporter assays, and in vivo xenograft models to confirm molecular interactions.
Main Results:
- Circ_0008344 and SZRD1 were upregulated, while miR-638 was downregulated in glioma tissues and cells.
- Circ_0008344 silencing suppressed glioma cell proliferation, migration, invasion, and angiogenesis.
- Circ_0008344 acted as a molecular sponge for miR-638, and the miR-638/SZRD1 axis mediated circ_0008344's effects on glioma.
Conclusions:
- Circ_0008344 promotes glioma progression and angiogenesis via the miR-638/SZRD1 pathway.
- Circ_0008344 represents a potential diagnostic biomarker and therapeutic target for glioma.
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