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Cpmer: A new conserved eEF1A2-binding partner that regulates Eomes translation and cardiomyocyte differentiation
Yao Lyu1, Wenwen Jia1, Yukang Wu1
1Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, National Stem Cell Translational Resource Center, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Stem Cell Reports
|April 8, 2022
Summary
A novel long noncoding RNA, Cpmer, regulates heart development by interacting with eukaryotic elongation factor 1A2 (eEF1A2). This interaction ensures proper translation of Eomes mRNA, crucial for cardiomyocyte differentiation and preventing congenital heart disease.
Area of Science:
- Cardiovascular Biology
- Molecular Genetics
- Developmental Biology
Background:
- Eukaryotic elongation factor 1A2 (eEF1A2) is vital for heart development, but its regulatory mechanisms are unclear.
- Mutations in eEF1A2 are linked to congenital heart disease and developmental delays.
- Understanding eEF1A2 regulation is key to addressing heart development disorders.
Purpose of the Study:
- To identify novel regulators of eEF1A2 function in mammalian heart development.
- To elucidate the molecular mechanisms by which eEF1A2 is regulated during cardiomyocyte differentiation.
- To explore the role of long noncoding RNAs (lncRNAs) in translational control during heart development.
Main Methods:
- Identification and characterization of the lncRNA Cpmer (cytoplasmic mesoderm regulator).
- Investigation of the interaction between Cpmer and eEF1A2 using biochemical and molecular assays.
- Analysis of Cpmer's role in Eomes mRNA recognition and translation.
- Assessment of Cpmer and eEF1A2 co-regulation of mouse and human embryonic stem cell-derived cardiomyocyte differentiation.
Main Results:
- Cpmer was identified as a lncRNA that interacts with eEF1A2 to co-regulate cardiomyocyte differentiation.
- Cpmer specifically binds to Eomes mRNA via RNA-RNA pairing.
- Cpmer facilitates eEF1A2 binding to Eomes mRNA, promoting its translation.
- This mechanism is conserved in both mouse and human cells, ensuring proper cardiomyocyte differentiation.
Conclusions:
- Cpmer is a novel, functionally conserved lncRNA that plays a critical role in mammalian heart development.
- Cpmer regulates cardiomyocyte differentiation by controlling Eomes mRNA translation through interaction with eEF1A2.
- This study reveals a new layer of translational regulation by lncRNAs in heart development, mediated by eEF1A2.
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