Related Experiment Video
Updated: Sep 27, 2025
Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
Published on: December 7, 2013
The Correlation between the Inflammatory Effects of Activated Macrophages in Atherosclerosis and Aortic Dissection
Xinbo Liu1, Jing Liu2, Youjin Li1
1Department of Cardiovascular Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, China.
Insights
Elevated lipid levels, inflammatory markers like TNF-α, IL-1β, IL-6, and M1 macrophage content are significantly linked to aortic dissection. Lower HDL and IL-10 levels were also observed in patients with aortic dissection.
Area of Science:
- Cardiovascular Research
- Immunology
- Biochemistry
Background:
- Aortic dissection is a life-threatening cardiovascular emergency.
- Understanding the underlying pathophysiological mechanisms is crucial for early detection and treatment.
Purpose of the Study:
- To investigate the correlation between lipid metabolism indices, inflammatory factors, and M1 macrophage content in patients with aortic dissection.
Main Methods:
- Blood samples were collected from patients with and without aortic dissection.
- Lipid profiles (TC, TG, LDL, HDL, ApoA1, ApoB1) and inflammatory markers (TNF-α, IL-1β, IL-6, IL-10) were quantified.
- M1 macrophage content was assessed using flow cytometry.
Main Results:
- Patients with aortic dissection exhibited higher levels of TC, TG, LDL, ApoB, TNF-α, IL-1β, and IL-6 compared to controls.
- Conversely, HDL, ApoA1, and IL-10 levels were lower in the aortic dissection group.
- A significantly higher proportion of M1 macrophages was found in patients with aortic dissection.
Conclusions:
- Lipid metabolism indices, specific inflammatory factors, and M1 macrophage content are significantly associated with the occurrence of aortic dissection.
- These factors may serve as potential biomarkers for predicting the risk of aortic dissection.
Background:
To study the correlation between lipid metabolism index, inflammatory factor index, and M1 macrophage content and aortic dissection.
Methods:
Patients with only basic atherosclerotic diseases were selected as the control group, and patients with only basic atherosclerotic diseases and aortic dissection were set as the experiment group. Blood of patients was collected. Chemiluminescent immunoassay was applied to determine the concentration of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), apolipoprotein A1 (ApoA1), and apolipoprotein B1 (ApoB1). Enzyme-linked immunosorbent assay was applied to measure the concentration of tumor necrosis factor-α (TNF-α), Interleukin 1β (IL-1β), interleukin-6 (IL-6), and interleukin-10 (IL-10). The content of M1 macrophages in blood was measured with the flow cytometry (FCM) method. The correlation between the inflammatory effects of activated macrophages in atherosclerosis and aortic dissection was analyzed by a simple linear regression analysis.
Results:
Concentration of TC, TG, LDL-C, and ApoB and the concentration of TNF-α, IL-1β, and IL-6 in the experiment group were markedly higher compared to those in the control group, whereas the concentration of HDL-C and ApoA1 and IL-10 concentration in the experiment group was markedly lower. The content of M1 macrophage in the control group was significantly lower compared to the experiment group. The proportion of M1 macrophages, concentration of TC, TG, LDL, HDL, ApoA1, and ApoB1, and concentration of TNF-α, IL-1β, IL-6, and IL-10 were all significantly correlated to the occurrence of aortic dissection.
Conclusions:
The proportion of M1 macrophages, concentration of TC, TG, LDL, HDL, ApoA1, and ApoB1, and concentration of TNF-α, IL-1β, IL-6, and IL-10 are significantly correlated with the occurrence of aortic dissection.
Related Concept Videos
01:38Inflammation
01:30Atherosclerosis I: Introduction
01:26Coronary Artery Disease II: Pathophysiology
01:21Myocarditis I: Introduction