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Updated: Sep 27, 2025
09:32
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
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MHC Class I Immunopeptidome: Past, Present, and Future.
1Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.
Molecular & Cellular Proteomics : MCP
|April 8, 2022
Summary
The immunopeptidome, peptides presented for immunosurveillance, has been extensively characterized over 35 years. Recent advances link it to the translatome, fueling a renaissance in antigen processing and T-cell cancer immunotherapy.
Area of Science:
- Immunology
- Molecular Biology
- Proteomics
Background:
- Major histocompatibility complex (MHC) class I and II molecules present short peptides, dictating T-cell recognition.
- Over 35 years, significant progress has been made in characterizing the immunopeptidome for immunosurveillance.
Purpose of the Study:
- To review major milestones in immunopeptidome characterization.
- To discuss the role of proteasome-mediated splicing in shaping the immunopeptidome.
- To detail recent findings connecting the immunopeptidome to the translatome via ribosome profiling (RiboSeq).
Main Methods:
- Review of historical milestones in immunopeptidome research.
- Discussion of proteasome-mediated splicing mechanisms.
- Integration of ribosome profiling (RiboSeq) data with immunopeptidome studies.
Main Results:
- Characterization of the immunopeptidome has advanced significantly.
- Proteasome-mediated splicing contributes to the immunopeptidome.
- Emerging links between the immunopeptidome and the translatome (RiboSeq) are identified.
Conclusions:
- The field of antigen processing is experiencing a renaissance.
- Continued progress requires new talent and integration of advanced techniques.
- T-cell-based cancer immunotherapy success drives interest in immunopeptidome research.
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