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Related Experiment Video

Updated: Sep 27, 2025

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

Antigen identification and high-throughput interaction mapping by reprogramming viral entry.

Connor S Dobson^1,2, Anna N Reich^1,2, Stephanie Gaglione^2,3

¹Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

|April 9, 2022

Summary

This summary is machine-generated.

Researchers developed a new method using targeted retroviruses to simultaneously analyze immune receptor repertoires and antigens. This breakthrough aids in understanding diseases and developing vaccines and immunotherapies.

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Last Updated: Sep 27, 2025

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

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Published on: October 29, 2015

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

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Published on: March 22, 2016

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Area of Science:

Immunology and Virology
Molecular Biology and Genetic Engineering

Background:

Understanding immune recognition is crucial for disease research, vaccine development, and immunotherapy.
Current technologies struggle to simultaneously analyze complex adaptive immune receptor repertoires and diverse antigens.

Purpose of the Study:

To develop a novel technology for simultaneous screening of receptor-antigen interactions.
To enable high-throughput analysis of adaptive immune responses.

Main Methods:

Utilized viral pseudotyping and molecular engineering with targeted retroviruses.
Displayed antigens on lentivirus surfaces, encoding identity in the viral genome.
Employed antigen-specific viral infection of cells expressing T or B cell receptors for single-cell sequencing readout.

Main Results:

Successfully established a system for one-pot library-on-library interaction screens.
Enabled simultaneous readout of both antigen and receptor identities via single-cell sequencing.
Demonstrated a modular, scalable system compatible with various cell types.

Conclusions:

The developed retroviral system effectively pairs receptors with antigens.
This technology provides a powerful tool for studying immune recognition and developing new therapies.
Offers broad potential applications in immunology, vaccinology, and beyond.