Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Rapamycin Pharmacogenomic Approach for the Childhood Dementia Niemann-Pick C.

Journal of inherited metabolic disease·2026
Same author

Complex History of Organellar Introgression in <i>Nothofagus</i> Trees: Chloroplast and Mitochondrial Capture Facilitated by Natural Selection.

Ecology and evolution·2026
Same author

Lineage-Specific Adaptive Strategies Shape the Response of de Novo Lager Yeast Hybrids to High-Gravity Fermentation.

Microbial biotechnology·2026
Same author

A New Set of Optogenetic Switches in Yeast Based on the BcWCL1 Photoreceptor.

Yeast (Chichester, England)·2026
Same author

The Southernmost Known Population of the Monito Del Monte, <i>Dromiciops gliroides</i>.

Ecology and evolution·2026
Same author

Ecological specialization and admixture drive the genomic and phenotypic diversity in Yarrowia lipolytica.

Cell reports·2026

Related Experiment Video

Updated: Sep 27, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

2.6K

A Saccharomyces eubayanus haploid resource for research studies.

Jennifer Molinet1,2, Kamila Urbina2,3, Claudia Villegas1

  • 1ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), 7500574, Santiago, Chile.

Scientific Reports
|April 9, 2022
PubMed
Summary

Researchers created stable haploid strains of Saccharomyces eubayanus, the wild parent of lager yeast, using CRISPR-Cas9. These new strains are valuable tools for future molecular genetics research and evolutionary studies.

More Related Videos

Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

18.8K
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
10:08

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

17.4K

Related Experiment Videos

Last Updated: Sep 27, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

2.6K
Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

18.8K
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
10:08

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

17.4K

Area of Science:

  • Microbiology
  • Yeast Genetics
  • Evolutionary Biology

Background:

  • Saccharomyces eubayanus is the wild progenitor of lager yeast (S. pastorianus).
  • This wild yeast is ecologically significant but lacks extensive molecular genetics tools.
  • Previous studies identified three main lineages (PB-1, PB-2, PB-3) within S. eubayanus.

Purpose of the Study:

  • To generate stable haploid strains of S. eubayanus for enhanced genetic manipulation.
  • To facilitate future evolutionary, ecological, and genetic studies of this wild yeast.
  • To characterize the mating behavior of S. eubayanus haploid strains.

Main Methods:

  • CRISPR-Cas9 gene editing to delete the HO gene.
  • Tetrad micromanipulation to isolate haploid spores.
  • Phenotypic characterization under various conditions.
  • Genomic analysis for off-target mutations and structural variants.

Main Results:

  • A collection of stable haploid S. eubayanus strains from three lineages was successfully generated.
  • Haploid derivatives exhibited phenotypes similar to their parental strains.
  • Genomic analysis revealed a low frequency of off-target mutations and no structural variants in haploid spores.
  • S. eubayanus showed a preference for remaining haploid under liquid mating conditions, unlike S. cerevisiae.

Conclusions:

  • The developed haploid strains provide a valuable resource for yeast molecular genetics.
  • S. eubayanus exhibits distinct mating behavior compared to S. cerevisiae.
  • These haploid strains will significantly advance research into yeast evolution and ecology.