Related Experiment Video

Updated: Sep 27, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Corynespora cassiicola

Mingming Liu1,2, Xintao Liu1, Hui Zhao1

  • 1Institute of Plant Protection, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Zhengzhou, 450002, Henan, China.

Archives of Virology
|April 9, 2022
PubMed

Insights

A novel mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1), was identified in the plant pathogen Corynespora cassiicola. This discovery expands our understanding of fungal viruses and their potential impact on agriculture.

Area of Science:

  • Mycology
  • Virology
  • Plant Pathology

Background:

  • Corynespora cassiicola is a significant fungal pathogen impacting crop yields.
  • Mycoviruses, viruses that infect fungi, can influence host biology and pathogenicity.

Purpose of the Study:

  • To molecularly characterize a novel mycovirus isolated from Corynespora cassiicola.
  • To determine the genomic features and phylogenetic relationship of the newly discovered virus.

Main Methods:

  • Isolation and sequencing of the mycovirus genome.
  • Bioinformatic analysis of open reading frames (ORFs) and conserved domains.
  • Phylogenetic analysis based on viral RNA-dependent RNA polymerase (RdRp).

Main Results:

  • A novel positive-sense single-stranded RNA (+ssRNA) mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1), was identified.
  • CcFV1 possesses a 6491 nt genome with three ORFs, including genes encoding RNA-dependent RNA polymerase (RdRp) and a chromosome segregation ATPase (Smc) domain.
  • Phylogenetic analysis places CcFV1 within the proposed family "Fusariviridae", showing highest similarity to Setosphaeria turcica fusarivirus 1.

Conclusions:

  • CcFV1 represents the first fusarivirus discovered in Corynespora cassiicola.
  • The findings suggest CcFV1 is a novel member of the "Fusariviridae" family.
  • This discovery contributes to the understanding of mycovirus diversity and evolution.

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