Multifunctional Double-Layer Composite Hydrogel Conduit Based on Chitosan for Peripheral Nerve Repairing

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Neuroscience

Background:

• Peripheral nerve injury poses significant clinical challenges, with traditional hollow conduits showing limitations in cell migration and nutrient transport.
• Developing advanced nerve conduits is crucial for improving functional recovery after nerve damage.

Purpose of the Study:

• To synthesize and evaluate a novel double-layer composite hydrogel nerve conduit with enhanced properties for peripheral nerve repair.
• To investigate the efficacy of the conduit loaded with 7,8-dihydroxyflavone (DHF) in promoting nerve regeneration.

Main Methods:

• A double cross-linked hydrogel was synthesized using Schiff base reaction and hydrophobic interactions, featuring injectable, self-healing, and conductive properties.
• The conductive hydrogel was integrated into an electrodeposited chitosan conduit, forming a double-layer structure.
• The composite conduit loaded with DHF was tested in a rat sciatic nerve defect model.

Main Results:

• The double-layer hydrogel conduit demonstrated excellent degradability, biocompatibility, antioxidant activity, and supported Schwann cell proliferation.
• Significant promotion of sciatic nerve regeneration was observed compared to hollow chitosan conduits.
• The DHF-loaded conduit's repair efficacy was comparable to autologous nerve transplantation.

Conclusions:

• The developed multifunctional double-layer composite hydrogel nerve conduit shows great potential for peripheral nerve repair.
• This innovative biomaterial offers a promising alternative to current treatments for nerve defects.