Pseudomonas tumuqii sp. nov., isolated from greenhouse soil

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, named LAMW06^T, was isolated from greenhouse soil in Beijing, China. In the 16S rRNA gene sequence comparison, strain LAMW06^T had the highest similarity with Pseudomonas cuatrocienegasensis 1N^T. Phylogenetic analysis based on the 16S rRNA and three housekeeping gene sequences (gyrB, rpoB and rpoD) indicated that strain represented a member of the genus Pseudomonas. The genome sequence size of the isolate was 5.5 Mb, with a DNA G + C content of 63.5 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain LAMW06^T and closely related members of Pseudomonas borbori R-20821^T, Pseudomonas taeanensis MS-3^T and P. cuatrocienegasensis 1N^T were 90.9%, 82.4%, 81.5% and 43.0%, 25.9%, 24.6% respectively. The major fatty acids contained summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c), C_18:1 ω7c and C_16:0. The primary respiratory quinone was ubiquinone-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, six aminophospholipids, six phospholipids, one aminolipid and one glycolipid. According to the genotypic, phylogenetic and chemotaxonomic data, strain LAMW06^T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas tumuqii sp. nov. is proposed. The type strain is LAMW06^T (= GDMCC 1.2003^T = KCTC 72829^T).