Preparation of Clostridium septicum antigen for hyperimmunization of horses using a dialyzed culture

The preparation of toxic cultures of Clostridium septicum is described, using an apparatus with a straight dialysis tubing, where the medium is filled both into the nutrition and cultivation space of the apparatus. Using the cultivation to nutrition volume rate 1:2, mean titre of lethal antigen in filtrates 3.86 limes mortis per mL and 300 dosis lethalis minima per Lm was obtained in comparison with the values of 2.22 and 150 respectively in flask filtrates. Native filtrates of dialyzed cultures were better antigens for hyperimmunization of horses than the culture filtrates from flasks.