A bifunctional fluorescent probe based on PET & ICT for simultaneously recognizing Cys and H₂S in living cells

Most reported probes that respond to Cysteine (Cys) and Hydrogen sulfide (H₂S) can only identify one analyte, or they were interfered with homocysteine (Hcy) and glutathione (GSH) when recognizing Cys and H₂S. In addition, nitrobenzoxadiazole (NBD) ether, as one of thiols recognition sites, inevitably encounters the situation that Cys, GSH and H₂S cannot be distinguished on the same channel at the cellular level. In this work, by introducing NBD ether and NBD amine, we constructed a bifunctional fluorescent probe NJB for dual-site response to Cys and H₂S via PET & ICT processes. NJB has wonderful selectivity for identifying Cys and HS^-, with limits of detection as low as 58.4 nM and 81.1 nM, respectively. Interestingly, NJB has been successfully applied to detect Cys and HS^- in MCF-7 cells. Therefore, the probe that serves as a great tool for inquiring the physiological and pathological functions of Cys and H₂S in living cells is promising.