Related Experiment Video
Updated: Sep 27, 2025
07:30
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
7.7K
Highly sensitive visual colorimetric sensor for xanthine oxidase detection by using MnO2-nanosheet-modified gold
Yawen Lian1, Xiang Yuan1, Yandan Wang1
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Summary
This study introduces a sensitive colorimetric assay using GNP@MnO2 nanoparticles for detecting xanthine oxidase (XOD) activity. The method offers ultrasensitive detection of XOD and its inhibitors in complex systems.
Area of Science:
- Biochemistry
- Nanotechnology
- Analytical Chemistry
Background:
- Xanthine oxidase (XOD) is an enzyme implicated in various diseases.
- Sensitive detection methods for XOD activity are crucial for diagnosis and drug screening.
- Existing methods may lack the sensitivity required for early disease detection.
Purpose of the Study:
- To develop a highly sensitive colorimetric assay for determining xanthine oxidase (XOD) activity.
- To utilize GNP@MnO2 core-shell nanoparticles as a probe for ultrasensitive molecular detection.
- To evaluate the inhibitory effect of quercetin on XOD activity.
Main Methods:
- Construction of a GNP@MnO2 core-shell nanoparticle probe.
- Utilizing single particle detection (SPD) based dark field microscopy (DFM).
- Observing color changes in the probe due to H2O2 produced by XOD activity.
Main Results:
- The assay demonstrated a broad linear dynamic range (0.02-4 mU/mL) and a low detection limit (7.82 μU/mL).
- Single particle detection allowed for noticeable color change detection even with trace amounts of XOD.
- Quercetin showed a significant inhibitory effect on XOD activity.
Conclusions:
- The developed SPD-based colorimetric strategy provides a novel approach for ultrasensitive XOD detection.
- This method is more sensitive than traditional ensemble sample measurements.
- The assay offers new insights for detecting molecules in complex biological systems.
