Acetyl group assisted rapid intramolecular recognition of hydrogen peroxide: A novel promising approach for efficient...

As a vital biomolecule, hydrogen peroxide (H₂O₂) is involved in many physiological and pathological processes. Therefore, it is important to detect H₂O₂ in vivo conveniently and efficiently. In this paper, we report a new method of nucleophilic addition of H₂O₂ to the acetyl group to promote the rapid intramolecular reaction, which can be used to develop an efficient H₂O₂ probe. Based on this unique auxiliary recognition part, a fluorescent probe for H₂O₂ detection was designed and synthesized. This probe has the advantages of high sensitivity (limits of detection 7.0 × 10^-8 M or even lower.), fast response (within 3 min) and large Stokes shift (225 nm), which not only can monitor exogenous and endogenous H₂O₂ in cells but also successfully achieves the change of endogenous H₂O₂ level caused by drug sexual organ injury in zebrafish.