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Updated: Sep 27, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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Whole-genome sequence analysis through online web interfaces: a review.

A W A C W R Gunasekara1, L G T G Rajapaksha1, T L Tung2

  • 1Veterinary Medical Center and College of Veterinary Medicine, Jeonbuk National University, Jeonju 54596, Korea.

Genomics & Informatics
|April 11, 2022
PubMed
Summary

This study offers a guide to free online bioinformatics tools for analyzing bacterial whole-genome sequencing (WGS) data. These resources help researchers overcome the cost and technical barriers associated with genomic data analysis.

Keywords:
average nucleotide identitysonline web serverssingle nucleotide polymorphismsvirulence factorswhole-genome sequencing

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Genomics

Background:

  • Whole-genome sequencing (WGS) advancements enable microbial genome analysis.
  • WGS projects incur significant costs and require substantial effort, particularly in data analysis.
  • Many research institutions lack the bioinformatics infrastructure for effective genomic data analysis.

Purpose of the Study:

  • To provide researchers with a guide to accessible online bioinformatics tools.
  • To facilitate the analysis of bacterial whole-genome sequence data.
  • To reduce the financial and computational barriers in genomic research.

Main Methods:

  • Identification and compilation of publicly available web-based bioinformatics tools.
  • Evaluation of tools for analyzing bacterial whole-genome sequence data.
  • Categorization of tools based on their analytical capabilities.

Main Results:

  • A curated list of user-friendly, online bioinformatics tools for WGS data analysis is presented.
  • These web interfaces offer cost-effective alternatives to commercial software and high-performance computing.
  • The guide empowers researchers to analyze genomic data without extensive bioinformatics expertise or resources.

Conclusions:

  • Accessible online bioinformatics tools can democratize whole-genome sequencing data analysis for bacterial genomes.
  • This approach significantly lowers the barrier to entry for researchers with limited resources.
  • The study promotes wider utilization of WGS data in microbiology research.