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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Development of the Sexual Organs in the Embryo and Fetus01:15

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Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
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Testes: Histology01:27

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A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
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Zygotic Development And Stem Cell Formation01:10

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Oogenesis02:07

Oogenesis

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In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
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Related Experiment Video

Updated: Sep 27, 2025

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Sertoli cell and spermatogonial development in pigs.

Yi Zheng1, Qiang Gao1, Tianjiao Li1

  • 1Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Journal of Animal Science and Biotechnology
|April 11, 2022
PubMed
Summary

This study details pig testicular development, revealing Sertoli cell and spermatogonial patterns from birth to maturity. These findings enhance understanding of spermatogenesis in pigs, crucial for improving swine breeding practices.

Keywords:
PigSertoli cellSpermatogenesisSpermatogoniaTestis

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Animal Science

Background:

  • Spermatogenesis, the process of sperm generation, involves self-renewal and differentiation of spermatogonia (stem cells) within seminiferous tubules.
  • Sertoli cells are vital somatic cells supporting germ cell development, while spermatogonia are primitive male germ cells.
  • Porcine Sertoli cell and spermatogonial development remains less understood compared to mice.

Purpose of the Study:

  • To systematically investigate the developmental patterns of Sertoli cells and spermatogonia in pigs.
  • To characterize the progression of spermatogenesis from neonate to sexual maturity in Duroc boars.
  • To provide foundational knowledge for advancing porcine breeding and reproductive technologies.

Main Methods:

  • Collection of Duroc porcine testes across nine age points (7 to 210 days old).
  • Histological and immunohistochemical analyses of testis sections.
  • Immunofluorescence staining for key proteins (e.g., AMH, SOX9, DBA, UCHL1, VASA, KIT, Ki67, PCNA) to assess cell proliferation and development.
  • Immunostaining for β-catenin and ZO-1 to study blood-testis barrier formation.

Main Results:

  • Detailed characterization of spermatogenic cell and seminiferous tubule development in pigs.
  • Elucidation of proliferative activity and developmental trajectories for Sertoli cells and distinct spermatogonial subtypes.
  • Insights into the establishment and maturation of the blood-testis barrier during porcine testicular development.

Conclusions:

  • This study provides the first systematic, longitudinal investigation of Sertoli cell and spermatogonial development in pigs.
  • The findings significantly expand knowledge of porcine spermatogenesis and testicular maturation.
  • This research offers a theoretical basis for optimizing pig breeding and husbandry strategies.