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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

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Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

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Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
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Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Updated: Sep 27, 2025

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Vangl2 Promotes Hematopoietic Stem Cell Expansion.

Sarah Bouali1, Roxann Hétu-Arbour1, Célia Gardet1,2

  • 1Institut National de La Recherche Scientifique, INRS-Centre Armand-Frappier Santé Biotechnologie, Université du Québec, Laval, QC, Canada.

Frontiers in Cell and Developmental Biology
|April 11, 2022
PubMed
Summary

Vangl2 is crucial for hematopoietic stem cell (HSC) function. Deleting Vangl2 in mice impairs HSC expansion and recovery, revealing Vangl2 as a new regulator of hematopoiesis.

Keywords:
Vangl2bone marrow transplantshematopoietic recoveryhematopoietic stem cellswnt/planar cell polarity pathway

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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Hematopoietic stem cell (HSC) self-renewal and differentiation are critical for maintaining blood cell production.
  • Cell polarity, regulated by pathways like Wnt/polarity, influences HSC function.
  • The role of Vangl2, a Wnt/polarity pathway component, in hematopoiesis is currently unknown.

Purpose of the Study:

  • To investigate the function of Vangl2 in mouse hematopoiesis and HSC maintenance.
  • To determine the impact of Vangl2 deletion on HSC self-renewal, differentiation, and hematopoietic recovery.

Main Methods:

  • Utilized Vangl2-deficient mouse models.
  • Assessed HSC expansion, differentiation, and reconstitution capacity post-transplantation.
  • Analyzed cell cycle regulators (p16INK4a) and signaling pathway components (β-catenin) in Vangl2-deficient HSCs.

Main Results:

  • Deletion of Vangl2 in hematopoietic cells impaired HSC expansion and post-transplant recovery.
  • Vangl2-deficient mice exhibited splenomegaly and expansion of myeloid-biased progenitors.
  • Vangl2-deficient cells showed reduced ability to reconstitute bone marrow in serial transplants, indicating impaired self-renewal.
  • Aged Vangl2-deficient HSCs displayed elevated p16INK4a and active β-catenin.

Conclusions:

  • Vangl2 plays a significant role in regulating HSC function and hematopoiesis.
  • Impaired HSC self-renewal and expansion in Vangl2-deficient cells may be linked to altered cell cycle control and Wnt signaling.
  • Vangl2 is identified as a novel regulator of hematopoiesis.