Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

6.6K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
6.6K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

1.8K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.8K
T Cell Types and Functions01:24

T Cell Types and Functions

1.4K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.4K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

74.6K
Overview
74.6K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

5.0K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
5.0K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.2K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

iPSC-Derived iNK Progenitors Engraft and Generate NK Cells in Unconditioned and Autologous Immune Humanized Mice.

Cell proliferation·2026
Same author

Unraveling material characteristics of different pork belly cuts from raw to dish: a comparative study of texture and flavor.

NPJ science of food·2026
Same author

Corneal Stromal Microdots in Dry Eye Disease: Clinical Characterization and Associations With Corneal Nerve Parameters.

Translational vision science & technology·2026
Same author

Double-target responsive FRET sensor based on DNA tetrahedron for robust detection of liver fibrosis-related mRNAs in activated hepatic stellate cells.

Biosensors & bioelectronics·2026
Same author

Characterization of the aqueous humor microbiome in Posner-Schlossman syndrome: an exploratory metagenomic sequencing study.

Frontiers in medicine·2026
Same author

AI-driven gamification and metacognitive strategy development: a study of primary school English vocabulary learning from a self-regulated learning perspective.

Frontiers in psychology·2026

Related Experiment Video

Updated: Sep 27, 2025

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
08:04

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

12.0K

T cell regeneration: an update on progress and challenges.

Rongqun Guo1,2,3,4, Hongling Wu1,2,3,4, Juan Du1,2,3,4

  • 1CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Blood Science (Baltimore, Md.)
|April 11, 2022
PubMed
Summary

Generating T cells from stem cells offers a promising alternative to patient T cells for cancer therapy. However, challenges remain in creating functional, immunocompetent cells for clinical use.

Keywords:
Hematopoietic stem/progenitor cellsNotchPluripotent stem cellsT cell regeneration

More Related Videos

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.3K
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.3K

Related Experiment Videos

Last Updated: Sep 27, 2025

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
08:04

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

12.0K
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.3K
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

24.3K

Area of Science:

  • Immunology
  • Stem Cell Biology
  • Cancer Therapy

Background:

  • Patient peripheral blood-derived T cells (PBT) show promise in treating blood cancers but face limitations like high cost and exhaustion.
  • Alternative T cell sources are needed due to the drawbacks of PBT, including availability and disease-dependency.
  • Pluripotent stem cells (PSC) offer a theoretical advantage for generating T cells, providing an unlimited source and gene-editing feasibility.

Purpose of the Study:

  • To review recent advancements in T cell regeneration from alternative sources.
  • To highlight the challenges hindering the clinical application of induced T cells.
  • To discuss strategies for overcoming technical barriers in T cell induction protocols.

Main Methods:

  • Review of current literature on T cell induction from pluripotent stem cells (PSC).
  • Analysis of protocols utilizing Notch ligands (DLL1/DLL4) for T cell differentiation.
  • Examination of emerging approaches like in vivo reprogramming and induced T cell precursor transplantation.

Main Results:

  • While PSC offer potential, straightforward protocols for functional T cell induction are still under development.
  • Existing T cell induction protocols have not yet translated to clinical applications due to technical barriers.
  • New strategies are emerging for in vivo T cell regeneration and precursor transplantation.

Conclusions:

  • Regenerating functional T cells from PSC remains a significant challenge for clinical translation.
  • Overcoming technical hurdles is crucial for developing effective 'off-the-shelf' T cell therapies.
  • Continued research into T cell regeneration is vital for advancing cancer immunotherapy.