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Related Experiment Video

Updated: Sep 27, 2025

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Sonoporation: Past, Present, and Future.

Joseph Rich1, Zhenhua Tian2, Tony Jun Huang3

  • 1Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.

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|April 11, 2022
PubMed
Summary
This summary is machine-generated.

Sonoporation, a physical cell membrane permeabilization technique, enables intracellular delivery for cell therapies. Novel non-bubble-based methods aim to improve cell viability and cargo effectiveness over traditional bubble-based approaches.

Keywords:
acousticsacoustofluidicsintracellular deliveryphysical permeabilizationsonoporation

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Intracellular delivery is crucial for advancing cell-based therapies and reprogramming.
  • Physical cell membrane permeabilization techniques offer versatile, high-throughput cargo delivery.
  • Sonoporation is a promising physical method for delivering various cargos into diverse cell types.

Purpose of the Study:

  • To review bubble-based and non-bubble-based sonoporation mechanisms for intracellular delivery.
  • To discuss technologies overcoming limitations of traditional sonoporation platforms.
  • To provide perspectives on future sonoporation mechanisms, technologies, and applications.

Main Methods:

  • Review of existing literature on sonoporation techniques.
  • Analysis of bubble-based and non-bubble-based sonoporation mechanisms.
  • Investigation of technological advancements in intracellular delivery.

Main Results:

  • Traditional bubble-based sonoporation faces challenges including reliance on contrast agents and potential cell damage.
  • Novel non-bubble-based sonoporation mechanisms are being developed to mitigate these limitations.
  • Sonoporation demonstrates broad applicability across different cell types and cargo molecules.

Conclusions:

  • Sonoporation is a key technology for intracellular delivery in cell therapies.
  • Advancements in non-bubble-based sonoporation promise enhanced cell viability and delivery efficiency.
  • Future research directions focus on refining sonoporation technologies and expanding their therapeutic applications.