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Updated: Sep 27, 2025

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Published on: June 12, 2020

Deconstructing Adipose Tissue Heterogeneity One Cell at a Time.

Dylan J Duerre¹, Andrea Galmozzi^1,2,3

¹Department of Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, United States.

Frontiers in Endocrinology

|April 11, 2022

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Adipose tissue, once viewed as simple fat storage, is now understood as a complex organ crucial for metabolic health. Recent single-cell studies reveal its intricate dynamics and role in systemic physiology.

Area of Science:

Adipose tissue biology
Metabolic homeostasis
Cellular dynamics

Background:

Adipose tissue is a central coordinator of physiologic metabolism.
Recent advances reveal its complex structure and composition.
Obesity and metabolic diseases are accelerating due to modern lifestyles and aging populations.

Purpose of the Study:

To synthesize the latest advances in adipose tissue biology.
To explore the impact of epigenetic mechanisms on adipose function.
To suggest future research directions for understanding adipose tissue's role in systemic physiology.

Main Methods:

Single-cell technologies for deep cell profiling.
Analysis of tissue and single-cell level data.
Review of current literature on adipose tissue dynamics.

Keywords:

adipose tissue beige adipocytes brown adipocytes obesity thermogenesis tissue heterogeneity type 2 diabetes white adipocytes

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Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Published on: October 20, 2019

Localization, Identification, and Excision of Murine Adipose Depots

Localization, Identification, and Excision of Murine Adipose Depots

Published on: December 4, 2014

Related Experiment Videos

Last Updated: Sep 27, 2025

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Published on: June 12, 2020

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Published on: October 20, 2019

Localization, Identification, and Excision of Murine Adipose Depots

Localization, Identification, and Excision of Murine Adipose Depots

Published on: December 4, 2014

Main Results:

Adipose tissue is a highly heterogeneous organ.
It plays major roles in metabolic homeostasis, endocrine signaling, and immune function.
Single-cell technologies have significantly advanced understanding of adipose tissue dynamics.

Conclusions:

Adipose tissue is critical for systemic physiology and metabolic health.
Epigenetic mechanisms significantly influence adipose function.
Further research using advanced technologies is needed to fully elucidate adipose tissue's role.