Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.2K
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

7.4K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysosomal lipid peroxidation contributes to ferroptosis induction via lysosomal membrane permeabilization.

Nature communications·2025
Same author

Mechanistic insights into drug supersaturation during lipid digestion in self-emulsifying drug delivery systems: A cryo-TEM and NMR study.

International journal of pharmaceutics·2025
Same author

Line-Shifting Triarylmethyl Radicals for Imaging of Enzyme Activity Using Overhauser-Enhanced Magnetic Resonance Imaging: Application to Alkaline Phosphatase.

Chemical & biomedical imaging·2025
Same author

Construction of a screening system for lipid-derived radical inhibitors and validation of hit compounds to target retinal and cerebrovascular diseases.

Redox biology·2024
Same author

The Early Pathogenesis of Diabetic Retinopathy and Its Attenuation by Sodium-Glucose Transporter 2 Inhibitors.

Diabetes·2024
Same author

Isolation and structural comparison of Ru<sup>II</sup>-dnp complexes [dnp = 2,6-bis-(1,8-naphthyridin-2-yl)pyridine] with axially or equatorially coordinating NCS ligands.

Acta crystallographica. Section E, Crystallographic communications·2022

Related Experiment Video

Updated: Sep 27, 2025

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K

Imaging analysis for multiple paramagnetic agents using OMRI and electrophoresis.

Ayano Enomoto1, Nao Kato2, Naomi Shirouzu2

  • 1Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859‑3298, Japan.

Journal of Clinical Biochemistry and Nutrition
|April 11, 2022
PubMed
Summary

This study introduces a novel method combining electrophoresis and Overhauser enhancement magnetic resonance imaging (OMRI) for analyzing paramagnetic contrast agents. The technique successfully separates and visualizes nitroxides in biological samples, aiding redox status analysis in diseases.

Keywords:
Overhauser enhanced MRIelectron paramagnetic resonancemultiple paramagnetic agentsseparation and analysis

More Related Videos

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.5K
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
17:16

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

Published on: December 9, 2010

10.4K

Related Experiment Videos

Last Updated: Sep 27, 2025

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

9.5K
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
17:16

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

Published on: December 9, 2010

10.4K

Area of Science:

  • Analytical Chemistry
  • Biomedical Imaging
  • Medical Diagnostics

Background:

  • Nitroxides are valuable molecular probes for studying disease models.
  • Accurate analysis of paramagnetic contrast agents is crucial for disease diagnostics.

Purpose of the Study:

  • To develop a simple analytical method for separating and semi-quantifying multiple paramagnetic contrast agents.
  • To apply this method for in vitro measurement of biological samples to determine redox status.

Main Methods:

  • Combining electrophoresis with Overhauser enhancement magnetic resonance imaging (OMRI).
  • Utilizing three nitroxides with varying ionic charges: 3-carbamoyl PROXYL, 3-carboxy PROXYL, and CAT-1.
  • Testing the method's applicability on biological samples, including whole blood.

Main Results:

  • Successful separation of nitroxides based on their ionic charge using electrophoresis.
  • Visualization of separated nitroxides via OMRI post-electrophoresis.
  • Demonstration that vehicle media like whole blood do not interfere with the analysis or OMRI enhancement.

Conclusions:

  • The developed method offers a straightforward approach for analyzing paramagnetic agents in biological samples.
  • This technique enables in vitro determination of redox status in specific tissue layers without extensive preprocessing.
  • The method holds promise for detailed analysis of redox-related diseases.