Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alkaline proteinase localization in myoblasts.

W T Stauber, V K Fritz, B Dahlmann

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    2021 Canadian Surgery Forum: Virtual, online Sept. 21-24, 2021.

    Canadian journal of surgery. Journal canadien de chirurgie·2022
    Same author

    Predictors of Poor Functional Status in Adult Fontan Patients Living at Moderate Altitude.

    Pediatric cardiology·2021
    Same author

    Healing of erosions in rheumatoid arthritis remains elusive: results with 24 months of the anabolic agent teriparatide.

    Scandinavian journal of rheumatology·2020
    Same author

    Impact of immunogenicity on efficacy and tolerability of tumour necrosis factor inhibitors: pooled analysis of biosimilar studies in rheumatoid arthritis.

    Scandinavian journal of rheumatology·2020
    Same author

    Correction to: PANLAR consensus statement on biosimilars.

    Clinical rheumatology·2019
    Same author

    PANLAR consensus statement on biosimilars.

    Clinical rheumatology·2019

    This study used immunohistochemistry to locate three alkaline proteases in rat myoblasts. Researchers confirmed the presence of trypsin-like, chymotrypsin-like, and cysteine proteinases within muscle cells.

    Area of Science:

    • Muscle physiology
    • Cellular biology
    • Biochemistry

    Background:

    • Non-lysosomal proteases play a role in intracellular protein breakdown in muscle.
    • Three alkaline proteases (trypsin-like, chymotrypsin-like, and high molecular weight cysteine proteinase) have been investigated.
    • Confirming the presence of these proteases within muscle cells has been challenging.

    Purpose of the Study:

    • To localize three specific alkaline proteases within rat myoblasts using immunohistochemical techniques.
    • To confirm the cellular location of trypsin-like, chymotrypsin-like, and cysteine proteinases in muscle cells.

    Main Methods:

    • Immunohistochemical techniques were employed to localize proteases in rat myoblasts.
    • Antisera against trypsin-like and chymotrypsin-like proteinases (serine proteinases) were used.

    Related Experiment Videos

  • Fluorescent localization was used for the high molecular weight cysteine proteinase (Proteinase I).

    • Main Results:

    • Trypsin-like and chymotrypsin-like serine proteinases were localized in the cytoplasm around the nucleus and also stained nuclear chromatin.
    • High molecular weight cysteine proteinase (Proteinase I) showed cell-associated localization in myoblasts.
    • Using myoblasts in cell culture facilitated protease localization assessment, free from interference by other cell types.

    Conclusions:

    • Immunohistochemistry successfully localized three key alkaline proteases within rat myoblasts.
    • The study confirms the presence and cellular distribution of these proteases in muscle cells.
    • Myoblast cell culture provides a suitable model for studying protease localization in muscle.