Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Norovirus-specific monoclonal antibodies that block histo-blood group antigen binding isolated from healthy donors.

Journal of virology·2026

Same author

A response to the Comment on "The effect of end-tidal carbon dioxide levels on dynamic cerebral autoregulation and clinical outcome in acute ischemic stroke: an INFOMATAS study".

Journal of the neurological sciences·2026

Same author

A longitudinal, qualitative exploration of women's sexual recovery following surgical repair of pelvic organ prolapse.

Women's health (London, England)·2026

Same author

Prefusion-specific glycoprotein B human antibodies protect against neonatal HSV-2 infection.

bioRxiv : the preprint server for biology·2026

Same author

The effect of end-tidal carbon dioxide levels on dynamic cerebral autoregulation and clinical outcome in acute ischemic stroke: An INFOMATAS study.

Journal of the neurological sciences·2026

Same author

Contrastive learning enables epitope overlap predictions for targeted antibody discovery.

Patterns (New York, N.Y.)·2026

Same journal

TLR7/8 agonist 3M-052 formulated in micelles induces local type I IFN response and antiviral immunity in zebrafish larvae.

Frontiers in immunology·2026

Same journal

HLA-DRB1*15:01 drives sex- and age-dependent microglial immune phenotypes and neuroimmune signaling.

Frontiers in immunology·2026

Same journal

Gut microbiota transfer from autoimmune dry eye mice imprints stereotypic B cell receptor repertoires in the lacrimal gland and induces disease.

Frontiers in immunology·2026

Same journal

Exosomal microRNAs in colorectal cancer communication networks: implications for metastasis, therapy resistance, and precision medicine.

Frontiers in immunology·2026

Same journal

TMEM160 promotes hepatocellular carcinoma cell proliferation, invasion, and immune evasion by regulating the VEGFA/PI3K/AKT signaling axis.

Frontiers in immunology·2026

Same journal

Nurr1 deficiency orchestrates a coupled liver-gut pathological axis revealed by multi-omics and deep-learning histopathology.

Frontiers in immunology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 27, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

High-Throughput B Cell Epitope Determination by Next-Generation Sequencing.

Lauren M Walker^1,2, Andrea R Shiakolas^1,2, Rohit Venkat¹

¹Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, United States.

Frontiers in Immunology

|April 11, 2022

Summary

This summary is machine-generated.

A new sequencing technology, LIBRA-seq with epitope mapping, efficiently identifies rare human monoclonal antibodies targeting specific epitopes. This method aids in discovering therapeutic antibodies against infectious diseases like HIV.

Keywords:

HIV epitope monoclonal antibody next generation sequencing (NGS)single cell

More Related Videos

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Related Experiment Videos

Last Updated: Sep 27, 2025

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Area of Science:

Immunology
Molecular Biology
Biotechnology

Background:

Monoclonal antibodies are crucial for combating infectious diseases.
Identifying antibodies with specific therapeutic epitopes is challenging with current methods.
Existing techniques often require extensive post-production characterization for epitope information.

Purpose of the Study:

To introduce LIBRA-seq with epitope mapping, a novel next-generation sequencing technology.
To enable high-throughput, residue-level epitope determination for single B cells simultaneously.
To accelerate the discovery of therapeutic antibodies targeting specific antigen sites.

Main Methods:

Development of LIBRA-seq with epitope mapping technology.
Utilizing an antigen panel of point mutants (HIV-1 Env glycoprotein).
Simultaneous epitope determination for thousands of single B cells.

Main Results:

Successfully identified and confirmed antibodies targeting multiple vulnerability sites on HIV-1 Env.
Demonstrated residue-level epitope mapping capabilities.
Validated the efficiency of LIBRA-seq for high-throughput antibody discovery.

Conclusions:

LIBRA-seq with epitope mapping is an efficient tool for identifying antibodies against specific epitopes.
This technology significantly advances the discovery of therapeutic monoclonal antibodies.
Enables rapid characterization of antibody-antigen interactions at a granular level.